Microbial Genome Sequencing Project
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Researcher(s):
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Major Lineage:
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Euryarchaeota
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NCBI taxID:
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439481
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Genome Size (Mb):
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2.0 Mb
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G+C %:
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39.1%
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Number of Genes:
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2932
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Coding %:
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86.1%
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Isolation Method:
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Isolated under sulfur reducing and heterotrophic conditions, pH 4.5, temp 70.Anaerobic marine media contained 300 ml of anaerobic MH buffer (87.75 gl-1 NaCl , 5.0 g l-1 MgCl2.6H2O, 1.5 g l-1 KCl2, 6.0 g l-1 NaOH) , (NH4)2SO4 (1.3 g l-1), KH2PO4(0.28 g l-1), MgSO4.7H2O (0.25 g l-1), CaCl2.2H2O (0.07 g l-1), FeCl3.6H2O (4 mg l-1),MnCl2.4H2O (0.36 mg), Na2B4O7 .10H2O (4.5 mg l-1), ZnSO4.7H2O (0.22 mg l-1),CuCl2.2H2O (0.05 mg l-1), Na2MoO4.2H2O (0.03 mg l-1), VoSO4.2H2O (0.03 mg), CoSO4 (0.01mg l-1), trisodium citrate (2.94 g l-1), trypticase peptone (1.0 g l-1), yeast extract (1.0 g l-1),resazurin (1.0 mg), Na2S.9H2O (0.50 g l-1), sulphur (10.0 g l-1). The pH was adjusted to 4.5 withH2SO4, and media was dispensed anaerobically under an atmosphere of N2:CO2 (80:20, v/v,100kPa).
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16S rRNA gene sequence:
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Status:
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Released to GenBank
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GenBank Accession Number:
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ABSD00000000
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Primary Citation:
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Reysenbach et al. Nature 442: 444-447.
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Relevance:
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Widely distributed at deep-sea vents- only information has been in clone libraries. This is the first isolate, and it's sister lineage are the Thermplasmales
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Location Collected:
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Mariner Vent field
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Depth (m):
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1920 m
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Latitude*:
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22° 10' 12" S
Convert to decimal
-22.17
Convert to degrees
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Longitude*:
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176° 36' 0" W
Convert to decimal
-176.6
Convert to degrees
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Location:
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| *In some cases, latitude and longitude are estimates based on information supplied by the researcher. |
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