Massachusetts Institute of Technology, Broad Institute
Environmental BAC and Fosmid sequencing
The Broad Institute will use this grant for the sequencing and automated assembly of 150 fosmids and 75 bacterial artificial chromosomes (outputs) to complement the Marine Microbiology Initiative’s metagenomics efforts. Several collaborators from the research community will submit environmental DNA samples from which, after passing quality control standards, clone libraries will be generated using the Broad Institute’s Genome Sequencing Platform. Raw sequence data will be submitted to an appropriate data repository, and sequence data will be assembled into contiguous DNA sequence strings and provided to the collaborators.