Monterey Bay Aquarium Research Institute
Development of marine eukaryotic metagenomic methods
The Monterey Bay Aquarium Research Institute will use this grant to support a collaborative team of researchers to address gaps in diversity and ecology of the eukaryotic microorganisms in the sea. Outputs include collection of three size classes of marine microorganisms, development of eukaryotic metagenomics methods, quality control experiments to assess sampling and sequencing biases, and a final synthesis of ecological and sequence data. This intensive molecular and environmental investigation not only requires the development of novel, validated eukaryotic metagenomic tools of broad use to the research community but also aims to decipher key ecological interactions among the larger and particle-attached marine eukaryotes, bacteria and archaea.